Multi-perspective scanning microscope based on Talbot effect
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چکیده
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منابع مشابه
Large - field - of - view , multi - perspective Talbot microscopy
Common microscope objectives have fields of view (FOVs) of less than a few millimeters because of limits imposed by optical aberrations. To scale up the FOVs, additional lens elements and heroic design efforts are required to compensate for the aberrations, leading to reduced transmissions and higher system costs. Such FOV limitations have thus become amajor bottleneck in microscopy for large-s...
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We demonstrate a long imaging depth, multi-perspective fluorescence scanning microscopy based on Talbot effect generated from a microlens array. An object with two layers that are 155μm apart was reconstructed from different perspectives. OCIS codes: (110.1758) Computational imaging; (180.2520) Fluorescence microscopy
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We have developed a focal plane tuning technique for use in focus-grid-based wide-field-of-view microscopy (WFM). In WFM, the incidence of a collimated beam on a mask with a two-dimensional grid of aperture produced the Talbot images of the aperture grid. The Talbot pattern functioned as a focus grid and was used to illuminate the sample. By scanning the sample across the focus grid and collect...
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Wide-field fluorescence microscopy is a commonly used imaging technique by researchers and clinicians. A standard wide-field microscope has no optical sectioning capabilities and this limits its use in imaging thick biological samples. Although standard wide-field fluorescence microscopy with deconvolution techniques can improve image quality [1], it does not provide true optical sectioning, du...
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Fluorescence Talbot microscope is a scalable field-of-view (FOV) imaging platform, which takes advantage of the phase sensitivity of the self-image of a periodic structure. Such a system can maintain the microscopic resolution and extend the FOV for the whole slide (15 mm × 15 mm) scanning. Previously reported Talbot fluorescence systems, tabletop and on-chip device alike, rely on the coherence...
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تاریخ انتشار 2016